MantisBT - MEGA
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0006844MEGAAlignment Explorerpublic2025-09-26 02:332025-09-26 15:13
guest 
gstecher 
normalminorhave not tried
resolvedno change required 
Windows11
MEGA 12 
 
Zoe
McFarlane
2469227M@student.gla.ac.uk
2469227M@student.gla.ac.uk
0006844: Access Violation
I am able to align my sequences using the Alignment Explorer, using the Muscle Alignment tool. I can save the file. I can then export the file in the MEGA format. However, when I try open this file MEGA main menu I get an "Access Violation" error message.
No tags attached.
? unaligned.mas (254,998) 2025-09-26 02:33
https://www.megasoftware.net/mantis_bt/
? exported.meg (260,766) 2025-09-26 02:33
https://www.megasoftware.net/mantis_bt/
Issue History
2025-09-26 02:33guestNew Issue
2025-09-26 02:33guestFile Added: unaligned.mas
2025-09-26 02:33guestFile Added: exported.meg
2025-09-26 15:13gstecherNote Added: 0008090
2025-09-26 15:13gstecherStatusnew => resolved
2025-09-26 15:13gstecherResolutionopen => no change required
2025-09-26 15:13gstecherAssigned To => gstecher

Notes
(0008090)
gstecher   
2025-09-26 15:13   
Hi Zoe,

I am writing in response to the bug report you recently submitted regarding the MEGA software. The error you encountered is caused by a double-quote character in the alignment in the "Zea Mays" sequence at position 2,745 (see attached). Removing this character before aligning will resolve the error. 

Best regards,
Glen Stecher
Institute for Genomics and Evolutionary Medicine
igem.temple.edu