MEGA X version 10.1 contains a number of enhancements over previous versions. They include
The RTDT (RelTime with Dated Tips) method for constructing timetrees calibrated using sample times from molecular data has been added.
MEGA now supports the use of statistical calibration densities (normal, lognormal, exponential, and uniform distributions) as divergence time constraints.
Users can calibrate RelTime trees using a strict molecular clock. A fixed evolutionary rate can be used when no divergence times are known or a single fixed divergence time can be used to set the molecular clock rate.
MEGA X now runs natively on macOS systems without the use of WINE.
A utility for calculating Evolutionary Probabilities (EP) has been added.
A method (CorrTest) for detecting auto-correlation of evolutionary rates in large phylogenies has been added.
A note about compatibility between different versions of MEGA X
ยท Session files (*.masx, *.mtsx, *mdsx) cannot be shared between different versions of MEGA X. For example, a tree session file (file.mtsx) created on a Linux system cannot be opened on a Windows computer. Additionally, a session file generated on a 32-bit operating system should not be opened on a 64-bit system and vice-versa.