Tamura-Nei gamma distance

 The Tamura-Nei (1993Tamura_and_Nei_1993) distance with the gamma model corrects for multiple hits, taking into account the different rates of substitution between nucleotides and the inequality of nucleotide frequencies.  In this distance, evolutionary rates among sites are modeled using the gamma distribution.  You will need to provide a gamma parameterRH_Gamma_parameter for computing this distance.

 

The Tamura-Nei model

 

MEGA provides facilities for computing the following quantities for this method:

Quantity

Description

d: Transitions & Transversions

Number of nucleotide substitutions per site.

s: Transitions only

Number of transitional substitutions per site.

v: Transversions only

Number of transversional substitutions per site.

R = s/v

Transition/transversions ratio.

L: No of valid common sites

Number of sites compared.

 

The formulas for computing these quantities are as follows:

Distances

where P1 and P2 are the proportions of transitional differences between nucleotides A and G, and between T and C, respectively, Q is the proportion of transversional differences, gA, gC, gG, gT, are the respective frequencies of A, C, G and T, gR = gA + gG, gY, = gT + gC, a is the gamma parameter and

Variances

where

 

See also Nei and Kumar (2000)Nei_and_Kumar_2000, page 45 and estimating gamma parameterHC_Computing_the_Gamma_Parameter.