Create a text file with coordinate information for all nsSNVs to be explored following the format below:
NP_000758 99 E
NP_000761 264 M
NP_000762 144 C
NP_000762 335 W
NP_000773 374 T
NP_000838 71 L
NP_000886 131 H
NP_000887 271 T
Each line contains coordinate information for one nsSNV and each value is separated by white space (i.e. spaces or tabs).
In the Mutation Explorer window, select File->Import Query Data From File (or click the upload data button) and browse for the newly created text file. MEGA-MD will first validate the format of the coordinate information file and then request prediction information for all specified nsSNVs from the MEGA-MDW web server. As data is retrieved, the Mutation Explorer window is updated.
The MEGA-MD application has no limit on the number of entries that can be included in the coordinate information file. However, depending on your internet connection speed and the current load on the MEGA-MDW server, retrieval of many predictions may take some time (anything less than 5,000 should not be problematic). For situations where MEGA-MD does not perform optimally due to high numbers of nsSNVs, the MEGA-MDW can be used directly ( www.mypeg.info/evod ). The same text file can be uploaded to the MEGA-MD server which will process the file and send you an email for retrieving prediction data once the processing is complete.