Main MEGA Window

The main window in MEGA contains a menu bar, a main toolbar (just beneath the menu bar), a secondary toolbar near the bottom of the window, and a bottom status bar.

Menu Bar

 

Menus: Description

File

Use the File menu commands to open data for analysis, edit text files, convert file formats, and exit MEGA.

Analysis

Use the Analysis menu to launch the analyses available in MEGA.

Help menu

Use the Help menu to access the online help system, which is displayed in a special help window.

 

Main Toolbar

This toolbar contains logically organized menus for launching the analyses available in MEGA as well as for importing/exporting data.

 

Align

Edit and build sequence alignments, view/edit sequencer files, query online data banks, do BLAST search, and launch the MEGA Web Browser.

Data

Open data and session files, explore active data, export active data, save active data to a session file, select genetic code table, select/edit genes and domains, select/edit taxa and groups.

Models

Launch analyses related to substitution models, such as best-fit model selection, pattern heterogeneity tests, estimation of substitution matrix and transition/transversion bias, calculate codon usage bias and composition statistics.

Distance

Compute evolutionary distances: pairwise, overall mean, within group mean, between group mean, and net between group mean.

Diversity

Compute mean diversity: within sub-populations, in entire population, between populations. Also, compute coefficient of differentiation.

Phylogeny

Construct/test phylogenies using Maximum Likelihood, Maximum Parsimony, Neighbor-Joining, Minimum Evolution, and UPGMA. Also, open saved tree sessions.

User Tree

Analyze a given tree using Maximum Likelihood, Maximum Parsimony, or Ordinary Least Squares. Display Newick trees, or edit/draw trees manually.

Ancestors

Infer ancestral states using Maximum Likelihood or Maximum Parsimony.

Selection

Estimate selection for each codon using HyPhy, perform codon-based Z-test of selection, codon-based Fisher’s exact test of selection, or Tajima’s test of neutrality.

Rates

Using Maximum Likelihood, estimate gamma shape parameter for site rates or estimate position-by-position rates.

Clocks

Perform Tajima’s relative rate test, test for molecular clock, or compute a time tree using the Reltime Maximum Likelihood method.

Diagnose

Explore the functional impact of non-synonymous single nucleotide variants (nSNVs).

 

 

Secondary Toolbar

This toolbar contains items that are not suitable for the main toolbar.

 

First time user?

A very brief description of using MEGA.

Tutorial

A collection of tutorials for learning how to perform common tasks when using MEGA.

Examples

A collection of example data files that are used in the tutorials.

Citation

How to cite MEGA.

Report a Bug

Help improve MEGA by filing a bug report with the authors.

Updates?

Check to see if there is a newer version of MEGA available.

MEGA Links

Links to resources related to MEGA.

Toolbar

Customize the main toolbar in MEGA.

Preferences

Customize user preferences.