The first line must contain the keyword #MEGA to indicate that the data file is in MEGA format. The data file may contain a succinct description of the data (called Title) included in the file on the second line. The Title statement is written according to a set of rules and is copied from MEGA to every output file. In the long run, an informative title will allow you to easily recognize your past work.
The data file may also contain a more descriptive multi-line account of the data in the Description statement, which is written after the Title statement. The Description statement also is written according to a set of rules. Unlike the Title statement, the Description statement is not copied from MEGA to every output file.
In addition, the data file may also contain a Format statement, which includes information on the type of data present in the file and some of its attributes. The Format statement should be generally written after the Title or the Description statement. Writing a format statement requires knowledge of the keywords used to identify different types of data and data attributes.
All taxa names must be written according to a set of rules.
Comments can be written anywhere in the data file and can span multiple lines. They must always be enclosed in square brackets ([and]) brackets and can be nested.