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| ID | Project | Category | View Status | Date Submitted | Last Update | ||||
| 0006834 | MEGA | Alignment Explorer | public | 2025-07-22 03:11 | 2025-07-28 10:37 | ||||
| Reporter | guest | ||||||||
| Assigned To | gstecher | ||||||||
| Priority | normal | Severity | minor | Reproducibility | have not tried | ||||
| Status | resolved | Resolution | won't fix | ||||||
| Platform | Windows | OS | 11 | ||||||
| Product Version | MEGA 12 | ||||||||
| Target Version | Fixed in Version | ||||||||
| Summary | 0006834: MUSCLE alignment can't find temporary file. | ||||||||
| Description | Can't perform MUSCLE alignments, due to the inability of MEGA to open a temporary file. | ||||||||
| Steps To Reproduce | Use supplied input .fas file, (unzip first), Open file, select Align, Align with MUSCLE, chose DNA, use default setting. See attached screen shot for error message. | ||||||||
| Tags | No tags attached. | ||||||||
| Attach Tags | (Separate by ",") | ||||||||
| First Name | Matthew | ||||||||
| Last Name | Bashton | ||||||||
| matthew.bashton@northumbria.ac.uk | |||||||||
| Confirm Email | matthew.bashton@northumbria.ac.uk | ||||||||
| Attached Files |  Screenshot 2025-07-22 110736.png (6,372 bytes) 2025-07-22 03:11 CoreGenome.zip (4,530,330 bytes) 2025-07-22 03:11 | ||||||||
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(0008080) gstecher (administrator) 2025-07-28 10:36 |
Hi Matthew, I am writing in response to the bug report you recently submitted regarding the MEGA software. Thank you for the feedback. The alignment process failed with your data due to an "out of memory" error. This happened because MEGA is using a 32-bit version of MUSCLE which cannot allocate sufficient memory for the alignment. A workaround is to download and use a more current version of MUSCLE - it can be downloaded at https://drive5.com/muscle/. [^] Alternatively, you could use MAFFT which is suitable for larger alignments. Best regards, Glen Stecher |
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(0008081) gstecher (administrator) 2025-07-28 10:37 |
In the future we may upgrade to newer MUSCLE. |
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Issue History |
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| Date Modified | Username | Field | Change |
| 2025-07-22 03:11 | guest | New Issue | |
| 2025-07-22 03:11 | guest | File Added: Screenshot 2025-07-22 110736.png | |
| 2025-07-22 03:11 | guest | File Added: CoreGenome.zip | |
| 2025-07-28 10:36 | gstecher | Note Added: 0008080 | |
| 2025-07-28 10:36 | gstecher | Status | new => resolved |
| 2025-07-28 10:36 | gstecher | Resolution | open => won't fix |
| 2025-07-28 10:36 | gstecher | Assigned To | => gstecher |
| 2025-07-28 10:37 | gstecher | Note Added: 0008081 | |
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Issue History |
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